Barcoding generalist predators by polymerase chain reaction: carabids and spiders.

نویسندگان

  • M H Greenstone
  • D L Rowley
  • U Heimbach
  • J G Lundgren
  • R S Pfannenstiel
  • S A Rehner
چکیده

Identification of arthropod predators is challenging when closely related species are found at a given locality. Identification of the immature stages is especially problematic, because distinguishing morphological features are difficult to use or have not been described. We used polymerase chain reaction (PCR) to distinguish closely related carabids and spiders, and to match eggs and larvae (or nymphs) with identified adult parents. Within the Carabidae, we amplified species-specific mitochondrial cytochrome oxidase I (COI) fragments for three species each in the genera Poecilus and Harpalus, and two each in Chlaenius and Bembidion. Within the Araneae, we amplified species-specific COI fragments for two Hibana species (Anyphaenidae), Pardosa milvina and Rabidosa rabida (Lycosidae), Frontinella communis and Grammonota texana (Linyphiidae), and Cheiracanthium inclusum (Miturgidae). We are able to correctly identify all immature stages tested--eggs, larvae (or nymphs) and pupae--by comparison of the amplified fragments with those of the adults. Using COI markers as species identifiers is a tenet of the Barcode of Life initiative, an international consortium to provide a molecular identifier for every animal species.

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Evaluation of temperature gradient gel electrophoresis for the analysis of prey DNA within the guts of invertebrate predators.

The utility of temperature gradient gel electrophoresis (TGGE) as a means of analysing the gut contents of predators was evaluated. Generalist predators consume multiple prey species and a species-specific primer approach may not always be a practical means of analysing predator responses to prey diversity in complex and biodiverse ecosystems. General invertebrate primers were used to amplify t...

متن کامل

Short Communication Spider Predation: Species-specific Identification of Gut Contents by Polymerase Chain Reaction

We extend detection of arthropod predator gut contents by polymerase chain reaction (PCR), heretofore restricted to insect predators, to spiders. Single individuals of the corn lead aphid, Rhopalosiphum maidis, were detected in the guts of spiderlings of Oxyopes salticus up to 12 h after feeding; individuals of the congeneric bird cherry oat aphid, R. padi, were not detected. Unfed O. salticus ...

متن کامل

Modular Habitat Refugia Enhance Generalist Predators and Lower Plant Damage in Soybeans

Promoting generalist predators in agriculture via habitat manipulation has gained much interest in biocontrol research. Straw shelters have been used by Chinese farmers for .2,000 yr to provide temporary spider refugia during cyclic farming disturbances. This method, however, has not been systematically investigated on larger scales in western-style agriculture. Our preliminary observations ind...

متن کامل

Secondary predation: quantification of food chain errors in an aphid-spider-carabid system using monoclonal antibodies.

"Secondary predation" occurs when one predator feeds on a second predator, which has in turn eaten a target prey. Detection of prey remains within predators using monoclonal antibodies cannot distinguish between primary and secondary predation, potentially leading to quantitative and qualitative food chain errors. We report the first fully replicated experiments to measure secondary predation e...

متن کامل

Analysis of the predator community of a subterranean herbivorous insect based on polymerase chain reaction.

The identity and impact of trophic linkages within subterranean arthropod communities are challenging to establish, a fact that hinders the development of conservation biological control programs of subterranean herbivores. Diabrotica virgifera (the western corn rootworm) is a severe agricultural pest that lives subterraneously during its pre-imaginal stages and succumbs to high levels of pre-i...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:
  • Molecular ecology

دوره 14 10  شماره 

صفحات  -

تاریخ انتشار 2005